Komet 5.5 software

By submitting this form I agree that Oxford Instruments will process my data in the manner described in the Privacy Policy. Your Question. Overview Features Resources Learning. Fast and easy to use — pop-up controls accelerate scoring and minimize fatigue - Proven performance — reliable data analysis with backward compatibility - User-friendly, freely distributed Database Viewer application - Databases include all comet images, parameters and audit trails - Integrated creation of summary statistics — optimizes data workflow and reporting - Facilitates OECD TG guideline requirements for data and statistical analysis - Fulfills FDA 21CFR part 11 requirements for electronic data - Certified Windows 7 compatibility - Windows 8.

Spec sheet. Komet Database: Helix3 - Pituitary. Komet Database: Helix3 - Adrenal. Comet assay:Quantifying DNA damage and repair at single cell level. You may also be interested in Latest news. Vendors and buyers have access to information in real time to facilitate and automate the procurement process. Business Intelligence. Develop a solid business strategy with multiple reports for the sales, procurement and administration department. Inventory Management. Manage your warehouse using labels, scanners, and reports to easily reconcile your inventory.

Integrations with Apps. The API allows you to integrate different systems to achieve synergy through the various company departments. Connecting with Our Customers. Your ideas to improve the system will be analyzed by our team of project managers and used to make Komet better for you! This method includes body temperature, tissue decomposition, insect succession and bone weathering Pounder By using the rate-of-change methodology, early TSD can be estimated on the basis of observational changes occur in human body after the death.

The cooling of the body after death algor mortis , the gradual stiffening of the body rigor mortis and the fixed pooling of the blood resulting in discoloration of the lower portions of the body livor mortis can be easily assessed with minimal instrumentation Clark et al. These Postmortem changes can roughly estimate the TSD but are unable to provide accurate estimation. Furthermore, these postmortem changes are very complex and influenced by the different internal and external factors Swift All of the above mentioned methods are effective but are insufficient to achieve the precise conclusion regarding to the TSD estimation.

Instead of independently, these observational changes can collectively predict well, but all these changes are influenced by cadaveric or environmental origin, so it may vary from case to case and person to person. Therefore, accurate TSD estimation is not possible with these methods.

Thus more accurate and reliable method for TSD estimation is urgently needed. In spite of the tremendous advancement in forensics and life sciences over the past few decades, especially through DNA analysis, TSD estimation is still lacking behind. DNA is a genetic material and present inside a nucleus of the cell, therefore external and internal factors would not be able to affect them easily. Previous studies have shown that DNA degradation and repair is mutual interchangeable biological processes occurring in the cells of living human bodies Reddy ; Clancy When any xenobiotic substance gets internalized into the cellular system, it may interact with genetic material such as DNA.

When they interact with DNA, conformational changes, single or double strand breaks, formation of alkali labile sites may occur Clancy When these changes are occurring in DNA, repair mechanism starts functioning immediately which is mainly regulating cell division process. These cell division processes stops in the cells, after death, then DNA repair pathway are impeded and DNA degradation process becomes un-repairable Clancy In this way, DNA degradation increase with prolongation of time of death and thus, this could be a good molecular clock for TSD estimation.

In this order, Xiong et al. This study revealed a positive linear correlation between the rate of DNA degradation and postmortem interval Xiong et al. In addition, forensic researchers have used a variety of techniques in this concern with different tissues as sources of samples which demonstrated the positive correlation between DNA degradation and postmortem interval Xiong et al.

In this order, forensic scientists have made an attempt to use flow cytometry technique for estimating postmortem interval through the assessment of DNA degradation Williams et al. But limitations to the procedure as it was originally proposed have proven difficult to overcome for forensic samples.

Flow cytometry requires a suspension for staining and analysis. This could make scientists difficult to analyze solid tissue without extensive manipulation. Several modifications to flow cytometry have been made due to which it become more specific and sensitive Goddard et al. Therefore, this technique is not commonly used for forensic purposes. Another point is, this instrument is not easily available, due to high cost.

For the forensic purposes we need a low cost, rapid, reliable, easily available and accessible technique that can be performed easily in the laboratory. This technique was first introduced by Ostling and Johnson , using a microgel electrophoresis technique to quantify DNA damage in cells. They have used this technique in the neutral conditions that only allow the detection of double-stranded DNA breaks. Later on, NP Singh et al.

This is a simple, inexpensive, and sensitive technique to test for DNA damage. Furthermore, Miteva et al. This study revealed that SCGE help to measure the kinetics of sperm DNA degradation which help to estimate the time since deposition of sperm or time of ejaculation of sperm.

With the help of this study, Miteva et al. Therefore, with the help of this paper an attempt was made to introduce SCGE assay, with its descriptive methodology and its role in the field of Forensic Science and also discuss about its future perspective. For semen sample, two different types of high resolution agarose gel are prepared which can be used for SCGE assay. Then add 1 ml of PBS in this clear solution and microwave the solution again.

End frosted microscopic slides are used for the SCGE assay. Slides are first dipped in methanol and burnt over a blue flame to remove machine oil and dust.

Wipe the underside of slide to remove agarose and lay the slide in a tray or on a flat surface to dry. The slides are stored in a dry slide box at room temperature until needed, and are generally prepared the day before use. In case of semen sample, slide is pre-coated with 0.

Thereafter, a microgel layer of agarose is prepared prior to put cells on the 0. Agarose is allowed to solidify at room temperature for 5 min or 1 min, if on ice. Before adding cell suspension onto the slide, cover slip is gently removed Simon et al. Thereafter, spermatozoa cells are collected through centrifuged and re-suspended in 1 ml PBS. This suspension is then mixed with 0.

Slide preparation is done according to a modified method of Tice et al. A cover-slip is placed on the slide to ensure that the gel is evenly spread, and the slide is kept on ice to allow the gel to solidify. Duplicate slides are prepared for each sample Bajpayee et al.

Again the slide is kept on ice to solidify the gel and the cover slip is finally removed to proceed for lysis Simon et al. This step helps to lyse the cells using lysing solution with detergent. Presence of high salt in lysing solution helps to remove cell membranes, bulk of proteins, cytoplasm and nucleoplasm. It further disrupts nucleosomes and almost all histones being solubilized by the high salt. Furthermore, it form nucleoids consisting of a nuclear matrix composed of ribonucleic acid RNA , proteins and containing negatively charged super coiled loops of DNA Collins ; Shukla et al.

In this step cover-slips are removed and the slides immersed in a freshly prepared and chilled lysis solution NOTE: The purpose of the DMSO in the lysing solution is to scavenge radicals generated by the iron released from hemoglobin when blood or tissues are used.

In case of semen sample, 0. With the help of this step, any breaks present in the DNA cause the super coiling to relax locally and loops of DNA are then free. This step proceeds after lysis of the cells embedded on base slide. The slides are left in this solution for DNA unwinding for 25 min. All steps are performed under dimmed light Shukla et al.